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CABRI:NCCB_PLASMID |
Collection_number |
NCCB 3000
|
Name |
pLV57
|
Other_culture_collection_numbers |
PC-V3000
|
Type |
plasmid
|
Class |
Recombinant
|
Literature |
O'Connor, C.D. et al., Gene 20 (1982) 219-229 [PMID: 6299889
] |
History_of_deposit |
-
|
Restricted_distribution |
No
|
Host_for_distribution |
Escherichia coli K12 HB101
|
Medium |
Medium 171
|
Selectable_phenotype |
Ampicillin resistance;Chloramphenicol resistance
|
Replicon |
pMB1
|
Transfer_ability |
Non-transferable
|
Host_range |
Escherichia coli vector
|
Properties_and_applications |
general cloning vector
|
Molecular_weight |
6.1 kb
|
Cloned_gene |
EcoRI
|
Further_information |
cells carrying this plasmid have to be grown at 30 C; expression of the EcoRI
gene is lethal to the cells at higher temperatures; recombinants do survive at 37 C
|
Restriction_sites |
(U,I(EcoRI)):BglII,HindIII; (U):AvaI,EcoRI
|
Price_code |
1
|
|